Oxidation dyeing composition for keratin fibers containing choline oxidase

ABSTRACT

The invention relates to a ready-to-use composition for the oxidation dyeing of keratin fibres, and in particular of human keratin fibres such as the hair, this composition comprising, in a medium which is suitable for dyeing, at least one oxidation base, choline oxidase, and at least one donor for the said choline oxidase, as well as to the dyeing process using this composition.

The invention relates to a composition for the oxidation dyeing ofkeratin fibres, and in particular of human keratin fibres such as thehair, this composition comprising, in a medium which is suitable fordyeing, at least one oxidation base, choline oxidase, and at least onedonor for the said choline oxidase, as well as to the dyeing processusing this composition.

It is known to dye keratin fibres, and in particular human hair, withdye compositions containing oxidation dye precursors, in particularortho- or para-phenylenediamines, ortho- or para-aminophenols andheterocyclic bases which are generally referred to as oxidation bases.Oxidation dye precursors, or oxidation bases, are colourless or weaklycoloured compounds which, when combined with oxidizing products, cangive rise to coloured compounds and dyes by a process of oxidativecondensation.

It is also known that the shades obtained with these oxidation bases canbe varied by combining them with couplers or colour modifiers, thelatter being chosen in particular from aromatic meta-diamines,meta-aminophenols, meta-diphenols and certain heterocyclic compounds.

The variety of compounds used as regards the oxidation bases and thecouplers allows a wide range of colours to be obtained.

The so-called "permanent" coloration obtained by means of theseoxidation dyes must moreover satisfy a certain number of requirements.Thus it must have no toxicological drawbacks, it must be able to giveshades of the desired intensity and it must be able to withstandexternal agents (light, bad weather, washing, permanent-waving,perspiration, rubbing).

The dyes must also be able to cover white hair and, lastly, they must beas unselective as possible, i.e. they must give the smallest possiblecolour differences along the same length of keratin fibre, which may infact be differently sensitized (i.e. damaged) between its tip and itsroot.

The oxidation dyeing of keratin fibres is generally carried out inalkaline medium, in the presence of hydrogen peroxide. However, the useof alkaline media in the presence of hydrogen peroxide has the drawbackof causing appreciable degradation of the fibres, as well asconsiderable bleaching of the keratin fibres, which is not alwaysdesirable.

The oxidation dyeing of keratin fibres can also be carried out usingoxidizing systems other than hydrogen peroxide, such as enzymaticsystems. Thus, it has already been proposed to dye keratin fibres, inparticular in patent application EP-A-0,310,675, with compositionscomprising an oxidation dye precursor of benzenic type in combinationwith enzymes such as pyranose oxidase, glucose oxidase or uricase, inthe presence of a donor for the said enzymes. Although being used underconditions which do not result in degradation of the keratin fibreswhich is comparable to that caused by the dyes used in the presence ofhydrogen peroxide, these dyeing processes are not entirely satisfactory,in particular as regards the strength of the colorations obtained.

Now, the Applicant has just discovered that it is possible to obtainnovel dyes, capable of leading to colorations that are stronger thanthose of the prior art using an enzymatic system, by combining at leastone oxidation base, at least one enzyme of choline oxidase type, and atleast one donor for the said enzyme.

This discovery forms the basis of the present invention.

A first subject of the invention is thus a ready-to-use composition forthe oxidation dyeing of keratin fibres, and in particular of humankeratin fibres such as the hair, characterized in that it comprises, ina medium which is suitable for dyeing:

at least one oxidation base,

choline oxidase,

and at least one donor for the said choline oxidase.

The dye composition in accordance with the invention makes it possibleto obtain colorations that are stronger than those obtained with thecompositions of the prior art using an enzymatic oxidizing system, suchas, for example, the uric acid/uricase oxidizing system.

Furthermore, the colorations obtained with the dye composition inaccordance with the invention are relatively unselective and show goodresistance to the various attacking factors to which the hair may besubjected (light, bad weather, washing, permanent-waving, etc.).

A subject of the invention is also a process for the oxidation dyeing ofkeratin fibres using this ready-to-use dye composition.

The nature of the oxidation base(s) used in the ready-to-use dyecomposition is not a critical factor. They can be chosen, in particular,from para-phenylenediamines, double bases, para-aminophenols,ortho-aminophenols and heterocyclic oxidation bases.

Among the para-phenylenediamines which can be used as oxidation bases inthe dye compositions in accordance with the invention, mention may bemade in particular of the compounds of formula (I) below, and theaddition salts thereof with an acid: ##STR1## in which: R₁ represents ahydrogen atom, a C₁ -C₄ alkyl radical, a C₁ -C₄ monohydroxyalkylradical, a C₂ -C₄ polyhydroxyalkyl radical, a (C₁ -C₄)alkoxy(C₁-C₄)alkyl radical, a C₁ -C₄ alkyl radical substituted with a nitrogenousgroup, a phenyl radical or a 4'-aminophenyl radical;

R₂ represents a hydrogen atom, a C₁ -C₄ alkyl radical, a C₁ -C₄monohydroxyalkyl radical, a C₂ -C₄ polyhydroxyalkyl radical, a (C₁-C₄)alkoxy(C₁ -C₄)alkyl radical or a C₁ -C₄ alkyl radical substitutedwith a nitrogenous group;

R₃ represents a hydrogen atom, a halogen atom such as a chlorine,bromine, iodine or fluorine atom, a C₁ -C₄ alkyl radical, a C₁ -C₄monohydroxyalkyl radical, a C₁ -C₄ hydroxyalkoxy radical, anacetylamino(C₁ -C₄)alkoxy radical, a C₁ -C₄ mesylaminoalkoxy radical ora carbamoylamino(C₁ -C₄)alkoxy radical,

R₄ represents a hydrogen or halogen atom or a C₁ -C₄ alkyl radical.

Among the nitrogenous groups of formula (I) above, mention may be madein particular of amino, mono(C₁ -C₄)alkylamino, di(C_(l) -C₄)alkylamino,tri(C₁ -C₄)alkylamino, monohydroxy(C₁ -C₄)alkylamino, imidazolinium andammonium radicals.

Among the para-phenylenediamines of formula (I) above, mention may bemade more particularly of para-phenylenediamine, para-toluylenediamine,2-chloro-para-phenylenediamine, 2,3-dimethyl-para-phenylenediamine,2,6-dimethyl-para-phenylenediamine, 2,6-diethyl-para-phenylenediamine,2,5-dimethyl-para-phenylenediamine, N,N-dimethyl-para-phenylenediamine,N,N-diethyl-para-phenylenediamine, N,N-dipropyl-para-phenylenediamine,4-amino-N,N-diethyl-3-methylaniline,N,N-bis(β-hydroxyethyl)-para-phenylenediamine,4-amino-N,N-bis(β-hydroxyethyl)-2-methylaniline,4-amino-2-chloro-N,N-bis(β-hydroxyethyl)aniline,2-β-hydroxyethyl-para-phenylenediamine, 2-fluoro-para-phenylenediamine,2-isopropyl-para-phenylenediamine,N-(β-hydroxypropyl)-para-phenylenediamine,2-hydroxymethyl-para-phenylenediamine,N,N-dimethyl-3-methyl-para-phenylenediamine,N,N-(ethyl-β-hydroxyethyl)-para-phenylenediamine,N-(β,γ-dihydroxypropyl)-para-phenylenediamine,N-(4'-aminophenyl)-para-phenylenediamine,N-phenyl-para-phenylenediamine,2-β-hydroxyethyloxy-para-phenylenediamine,2-β-acetylaminoethyloxy-para-phenylenediamine andN-(β-methoxyethyl)-para-phenylenediamine, and the addition salts thereofwith an acid.

Among the para-phenylenediamines of formula (I) above,para-phenylenediamine, para-toluylenediamine,2-isopropyl-para-phenylenediamine,2-β-hydroxyethyl-para-phenylenediamine,2-β-hydroxyethyloxy-para-phenylenediamine,2,6-dimethyl-para-phenylenediamine, 2,6-diethyl-para-phenylenediamine,2,3-dimethyl-para-phenylenediamine,N,N-bis(β-hydroxyethyl)-para-phenylenediamine,2-chloro-para-phenylenediamine and2-β-acetylaminoethyloxy-para-phenylenediamine and the addition saltsthereof with an acid are most particularly preferred.

According to the invention, the term "double bases" is understood torefer to the compounds containing at least two aromatic rings bearingamino and/or hydroxyl groups.

Among the double bases which can be used as oxidation bases in the dyecompositions in accordance with the invention, mention may be made inparticular of the compounds corresponding to formula (II) below, and theaddition salts thereof with an acid: ##STR2## in which: Z₁ and Z₂, whichmay be identical or different, represent a hydroxyl or --NH₂ radicalwhich may be substituted with a C₁ -C₄ alkyl radical or with a linkerarm Y;

the linker arm Y represents a linear or branched alkylene chaincontaining from 1 to 14 carbon atoms, which may be interrupted by orterminated with one or more nitrogenous groups and/or one or more heteroatoms such as oxygen, sulphur or nitrogen atoms, and optionallysubstituted with one or more hydroxyl or C₁ -C₆ alkoxy radicals;

R₅ and R₆ represent a hydrogen or halogen atom, a C₁ -C₄ alkyl radical,a C₁ -C₄ monohydroxyalkyl radical, a C₂ -C₄ polyhydroxyalkyl radical, aC₁ -C₄ aminoalkyl radical or a linker arm Y;

R₇, R₈, R₉, R₁₀, R₁₁ and R₁₂, which may be identical or different,represent a hydrogen atom, a linker arm Y or a C₁ -C₄ alkyl radical;

it being understood that the compounds of formula (II) contain only onelinker arm Y per molecule.

Among the nitrogenous groups of formula (II) above, mention may be madein particular of amino, mono(C₁ -C₄)alkylamino, di(C₁ -C₄)alkylamino,tri(C₁ -C₄)alkylamino, monohydroxy(C₁ -C₄)alkylamino, imidazolinium andammonium radicals.

Among the double bases of formula (II) above, mention may be made moreparticularly ofN,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)-1,3-diaminopropanol,N,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)ethylenediamine,N,N'-bis(4-aminophenyl)tetramethylenediamine,N,N'-bis(β-hydroxyethyl)-N,N'-bis(4-aminophenyl)tetramethylenediamine,N,N'-bis(4-methylaminophenyl)tetramethylenediamine,N,N'-bis-(ethyl)-N,N'-bis(4'-amino-3'-methylphenyl)ethylenediamine and1,8-bis(2,5-diaminophenoxy)-3,5-dioxaoctane, and the addition saltsthereof with an acid.

Among these double bases of formula (II),N,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)-1,3-diaminopropanoland 1,8-bis(2,5-diaminophenoxy)-3,5-dioxaoctane, or one of the additionsalts thereof with an acid, are particularly preferred.

Among the para-aminophenols which can be used as oxidation bases in thedye compositions in accordance with the invention, mention may be madein particular of the compounds corresponding to formula (III) below, andthe addition salts thereof with an acid: ##STR3## in which: R₁₃represents a hydrogen or halogen atom or a C₁ -C₄ alkyl, C₁ -C₄monohydroxyalkyl, (C₁ -C₄)alkoxy(C₁ -C₄)alkyl, C₁ -C₄ aminoalkyl orhydroxy(C₁ -C₄)alkylamino(C₁ -C₄)alkyl radical,

R₁₄ represents a hydrogen or halogen atom or a C₁ -C₄ alkyl, C₁ -C₄monohydroxyalkyl, C₂ -C₄ polyhydroxyalkyl, C₁ -C₄ aminoalkyl, C₁ -C₄cyanoalkyl or (C₁ -C₄)alkoxy(C₁ -C₄)alkyl radical,

it being understood that at least one of the radicals R₁₃ or R₁₄represents a hydrogen atom.

Among the para-aminophenols of formula (III) above, mention may be mademore particularly of para-aminophenol, 4-amino-3-methylphenol,4-amino-3-fluorophenol, 4-amino-3-hydroxymethylphenol,4-amino-2-methylphenol, 4-amino-2-hydroxymethylphenol,4-amino-2-methoxymethylphenol, 4-amino-2-aminomethylphenol,4-amino-2-(β-hydroxyethylaminomethyl)phenol and 4-amino-2-fluorophenol,and the addition salts thereof with an acid.

Among the ortho-aminophenols which can be used as oxidation bases in thedye compositions in accordance with the invention, mention may be mademore particularly of 2-aminophenol, 2-amino-5-methylphenol,2-amino-6-methylphenol and 5-acetamido-2-aminophenol, and the additionsalts thereof with an acid.

Among the heterocyclic bases which can be used as oxidation bases in thedye compositions in accordance with the invention, mention may be mademore particularly of pyridine derivatives, pyrimidine derivatives,pyrazole derivatives and pyrazolopyrimidine derivatives, and theaddition salts thereof with an acid.

Among the pyridine derivatives, mention may be made more particularly ofthe compounds described, for example, in patents GB 1,026,978 and GB1,153,196, such as 2,5-diaminopyridine,2-(4-methoxyphenyl)amino-3-aminopyridine, 2,3-diamino-6-methoxypyridine,2-(β-methoxyethyl)amino-3-amino-6-methoxypyridine and3,4-diaminopyridine, and the addition salts thereof with an acid.

Among the pyrimidine derivatives, mention may be made more particularlyof the compounds described, for example, in German patent DE 2,359,399or Japanese patents JP 88-169,571 and JP 91-333,495, or patentapplication WO 96/15765, such as 2,4,5,6-tetra-aminopyrimidine,4-hydroxy-2,5,6-triaminopyrimidine, 2-hydroxy-4,5,6-triaminopyrimidine,2,4-dihydroxy-5,6-diaminopyrimidine and 2,5,6-triaminopyrimidine, andthe addition salts thereof with an acid.

Among the pyrazole derivatives, mention may be made more particularly ofthe compounds described in patents DE 3,843,892, DE 4,133,957 and patentapplications WO 94/08969, WO 94/08970, FR-A-2,733,749 and DE 195 43 988,such as 4,5-diamino-1-methylpyrazole, 3,4-diaminopyrazole,4,5-diamino-1-(4'-chlorobenzyl)pyrazole,4,5-diamino-1,3-dimethylpyrazole, 4,5-diamino-3-methyl-1-phenylpyrazole,4,5-diamino-1-methyl-3-phenylpyrazole,4-amino-1,3-dimethyl-5-hydrazinopyrazole,1-benzyl-4,5-diamino-3-methylpyrazole,4,5-diamino-3-tert-butyl-1-methylpyrazole,4,5-diamino-1-tert-butyl-3-methylpyrazole,4,5-diamino-1-(β-hydroxyethyl)-3-methylpyrazole,4,5-diamino-1-ethyl-3-methylpyrazole,4,5-diamino-1-ethyl-3-(4'-methoxyphenyl)pyrazole,4,5-diamino-1-ethyl-3-hydroxymethylpyrazole,4,5-diamino-3-hydroxymethyl-1-methylpyrazole,4,5-diamino-3-hydroxymethyl-1-isopropylpyrazole,4,5-diamino-3-methyl-1-isopropylpyrazole,4-amino-5-(2'-aminoethyl)amino-1,3-dimethylpyrazole,3,4,5-triaminopyrazole, 1-methyl-3,4,5-triaminopyrazole,3,5-diamino-1-methyl-4-methylaminopyrazole and3,5-diamino-4-(β-hydroxyethyl)amino-1-methylpyrazole, and the additionsalts thereof with an acid.

Among the pyrazolopyrimidine derivatives, mention may be made moreparticularly of the pyrazolo[1,5-a]pyrimidines of formula (IV) below,and the addition salts thereof with an acid or with a base and thetautomeric forms thereof, when a tautomeric equilibrium exists: ##STR4##in which: R₁₅, R₁₆, R₁₇ and R₁₈, which may be identical or different,denote a hydrogen atom, a C₁ -C₄ alkyl radical, an aryl radial, a C₁ -C₄hydroxyalkyl radical, a C₂ -C₄ polyhydroxyalkyl radical, a (C₁-C₄)alkoxy(C₁ -C₄)alkyl radical, a C₁ -C₄ aminoalkyl radical (it beingpossible for the amine to be protected with an acetyl, ureido orsulphonyl radical), a (C₁ -C₄)alkylamino(C₁ -C₄)alkyl radical, adi[(C_(l) -C₄)alkyl]amino(C₁ -C₄)alkyl radical (it being possible forthe dialkyl radicals to form a 5- or 6-membered carbon-based ring orheterocycle), a hydroxy(C₁ -C₄)alkyl- or di[hydroxy(C₁-C₄)alkyl]amino(C₁ -C₄)alkyl radical;

the radicals X, which may be identical or different, denote a hydrogenatom, a C₁ -C₄ alkyl radical, an aryl radical, a C₁ -C₄ hydroxyalkylradical, a C₂ -C₄ polyhydroxyalkyl radical, a C₁ -C₄ aminoalkyl radical,a (C₁ -C₄)alkylamino(C₁ -C₄)alkyl radical, a di[(C₁ -C₄)alkyl]amino(C₁-C₄)alkyl radical (it being possible for the dialkyls to form a 5- or6-membered carbon-based ring or heterocycle), a hydroxy(C₁ -C₄)alkyl- ordi[hydroxy(C₁ -C₄)alkyl]amino(C₁ -C₄)alkyl radical, an amino radical, a(C₁ -C₄)alkyl- or di[(C₁ -C₄)alkyl]amino radical; a halogen atom, acarboxylic acid group or a sulphonic acid group;

i is equal to 0, 1, 2 or 3;

p is equal to 0 or 1;

q is equal to 0 or 1;

n is equal to 0 or 1;

with the proviso that:

the sum p+q is other than 0;

when p+q is equal to 2, then n is equal to 0 and the groups NR₁₅ R₁₆,and NR₁₇ R₁₈ occupy the (2,3); (5,6); (6,7); (3,5) or (3,7) positions;

when p+q is equal to 1, then n is equal to 1 and the group NR₁₅ R₁₆ (orNR₁₇ R₁₈) and the OH group occupy the (2,3); (5,6); (6,7); (3,5) or(3,7) positions.

When the pyrazolo[1,5-a]pyrimidines of formula (IV) above are such thatthey contain a hydroxyl group on one of the positions 2, 5 or 7 α to anitrogen atom, a tautomeric equilibrium exists represented, for example,by the following scheme: ##STR5##

Among the pyrazolo[1,5-a]pyrimidines of formula (IV) above, mention maybe made in particular of:

pyrazolo[1,5-a]pyrimidine-3,7-diamine;

2,5-dimethylpyrazolo[1,5-a]pyrimidine-3,7-diamine;

pyrazolo[1,5-a]pyrimidine-3,5-diamine;

2,7-dimethylpyrazolo[1,5-a]pyrimidine-3,5-diamine;

3-aminopyrazolo[1,5-a]pyrimidin-7-ol;

3-aminopyrazolo[1,5-a]pyrimidin-5-ol;

2-(3-aminopyrazolo[1,5-a]pyrimidin-7-ylamino)ethanol;

2-(7-aminopyrazolo[1,5-a]pyrimidin-3-ylamino)ethanol;

2-[(3-aminopyrazolo[1,5-a]pyrimidin-7-yl)-(2-hydroxyethyl)amino]ethanol;

2-[(7-aminopyrazolo[1,5-a]pyrimidin-3-yl)-(2-hydroxyethyl)amino]ethanol;

5,6-dimethylpyrazolo[1,5-a]pyrimidine-3,7-diamine;

2,6-dimethylpyrazolo[1,5-a]pyrimidine-3,7-diamine;

2,5,N7,N7-tetramethylpyrazolo[1,5-a]pyrimidine-3,7-diamine;

and the addition salts thereof and the tautomeric forms thereof, when atautomeric equilibrium exists.

The pyrazolo[1,5-a]pyrimidines of formula (IV) above can be prepared bycyclization starting with an aminopyrazole, according to the synthesesdescribed in the following references:

EP 628559 Beiersdorf-Lilly.

R. Vishdu, H. Navedul, Indian J. Chem., 34b (6), 514, 1995.

N. S. Ibrahim, K. U. Sadek, F. A. Abdel-Al, Arch. Pharm., 320, 240,1987.

R. H. Springer, M. B. Scholten, D. E. O'Brien, T. Novinson, J. P.Miller, R. K. Robins, J. Med. Chem., 25, 235, 1982.

T. Novinson, R. K. Robins, T. R. Matthews, J. Med. Chem., 20, 296, 1977.

US 3907799 ICN Pharmaceuticals.

The pyrazolo[1,5-a]pyrimidines of formula (IV) above can also beprepared by cyclization starting from hydrazine, according to thesyntheses described in the following references:

A. McKillop and R. J. Kobilecki, Heterocycles, 6(9), 1355, 1977.

E. Alcade, J. De Mendoza, J. M. Marcia-Marquina, C. Almera, J. Elguero,J. Heterocyclic Chem., 11(3), 423, 1974.

K. Saito, I. Hori, M. Higarashi, H. Midorikawa, Bull. Chem. Soc. Japan,47(2), 476, 1974.

The oxidation base(s) in accordance with the invention preferablyrepresent(s) from 0.0005 to 12% by weight approximately relative to thetotal weight of the ready-to-use dye composition, and even morepreferably from 0.005 to 6% by weight approximately relative to thisweight.

The choline oxidase used in the ready-to-use dye composition inaccordance with the invention can be of animal, microbiological(bacterial, fungal or viral) or synthetic (obtained by chemical orbiotechnological synthesis) origin.

As examples of sources of choline oxidase, mention may be made inparticular of rat liver, bacteria such as Arthrobacter globiformis,Achromobacter cholinophagum or Alcaligenes, and fungi such asCylindrocarpon didynum.

The choline oxidase used in the ready-to-use dye composition inaccordance with the invention preferably represents from 0.01 to 20% byweight approximately relative to the total weight of the ready-to-usedye composition, and even more preferably from 0.1 to 5% by weightapproximately relative to this weight.

According to the invention, the term "donor" refers to the differentsubstrate(s) required for the functioning of the choline oxidase.

Among the donors for choline oxidase, mention may be made of choline andits addition salts with an acid, such as choline hydrochloride, andbetaine aldehyde.

The donor(s) (or substrates) used in accordance with the inventionpreferably represent(s) from 0.01 to 20% by weight approximatelyrelative to the total weight of the ready-to-use dye composition inaccordance with the invention, and even more preferably from 0.1 to 5%approximately relative to this weight.

The ready-to-use dye composition in accordance with the invention canalso contain one or more couplers and/or one or more direct dyes, inparticular in order to modify the shades or to enrich them with glints.

Among the couplers which can be used in the ready-to-use dyecompositions in accordance with the invention, mention may be made inparticular of meta-phenylenediamines, meta-aminophenols, meta-diphenolsand heterocyclic couplers such as, for example, indole derivatives,indoline derivatives, benzimidazole derivatives, benzomorpholinederivatives, sesamol derivatives and pyridine, pyrimidine and pyrazolederivatives, and the addition salts thereof with an acid.

These couplers can be chosen more particularly from2-methyl-5-aminophenol, 5-N-(β-hydroxyethyl)amino-2-methylphenol,3-aminophenol, 1,3-dihydroxybenzene, 1,3-dihydroxy-2-methylbenzene,4-chloro-1,3-dihydroxybenzene, 2,4-diamino-1-(β-hydroxyethyloxy)benzene,2-amino-4-(β-hydroxyethylamino)-1-methoxybenzene, 1,3-diaminobenzene,1,3-bis(2,4-diaminophenoxy)propane, sesamol, α-naphthol,6-hydroxyindole, 4-hydroxyindole, 4-hydroxy-N-methylindole,6-hydroxyindoline, 2,6-dihydroxy-4-methylpyridine,1H-3-methylpyrazol-5-one and 1-phenyl-3-methylpyrazol-5-one, and theaddition salts thereof with an acid.

When they are present, these couplers preferably represent from 0.0001to 10% by weight approximately relative to the total weight of theready-to-use dye composition, and even more preferably from 0.005 to 5%by weight approximately relative to this weight.

In general, the addition salts with an acid which can be used in thecontext of the dye compositions of the invention (oxidation bases andcouplers) are chosen in particular from the hydrochlorides,hydrobromides, sulphates, tartrates, lactates and acetates.

The medium which is suitable for dyeing (or the support) for theready-to-use dye composition in accordance with the invention generallyconsists of water or of a mixture of water and at least one organicsolvent in order to dissolve the compounds which would not besufficiently soluble in water. By way of organic solvent, mention may bemade, for example, of C₁ -C₄ alkanols such as ethanol and isopropanol;glycerol; glycols and glycol ethers such as 2-butoxyethanol, propyleneglycol, propylene glycol monomethyl ether, diethylene glycol monoethylether and monomethyl ether, and aromatic alcohols such as benzyl alcoholor phenoxyethanol, similar products and mixtures thereof.

The solvents can be present in proportions preferably of between 1 and40% by weight approximately relative to the total weight of the dyecomposition, and even more preferably between 5 and 30% by weightapproximately.

The pH of the ready-to-use composition in accordance with the inventionis chosen such that the enzymatic activity of the choline oxidase issufficient. It is generally between 5 and 11 approximately, andpreferably between 6.5 and 10 approximately. It can be adjusted to thedesired value using acidifying or basifying agents usually used fordyeing keratin fibres.

Among the acidifying agents, mention may be made, by way of example, ofinorganic or organic acids such as hydrochloric acid, orthophosphoricacid, sulphuric acid, carboxylic acids such as acetic acid, tartaricacid, citric acid or lactic acid, and sulphonic acids.

Among the basifying agents, mention may be made, by way of example, ofaqueous ammonia, alkaline carbonates, alkanolamines such as mono-, di-and triethanolamines, 2-methyl-2-aminopropanol and derivatives thereof,sodium hydroxide, potassium hydroxide and the compounds of formula (V)below: ##STR6## in which W is a propylene residue optionally substitutedwith a hydroxyl group or a C₁ -C₄ alkyl radical; R₁₉, R₂₀, R₂₁ and R₂₂,which may be identical or different, represent a hydrogen atom or a C₁-C₄ alkyl or C_(1-C) ₄ hydroxyalkyl radical.

The ready-to-use dye composition in accordance with the invention canalso contain various adjuvants used conventionally in compositions fordyeing the hair, such as anionic, cationic, nonionic, amphoteric orzwitterionic surfactants or mixtures thereof, anionic, cationic,nonionic, amphoteric or zwitterionic polymers or mixtures thereof,inorganic or organic thickeners, antioxidants, enzymes other than thecholine oxidase used in accordance with the invention, such as, forexample, peroxidases, penetration agents, sequestering agents,fragrances, buffers, dispersing agents, conditioners such as, forexample, volatile or non-volatile, modified or non-modified silicones,film-forming agents, ceramides, preserving agents and opacifiers.

Needless to say, a person skilled in the art will take care to selectthis or these optional complementary compound(s) such that theadvantageous properties intrinsically associated with the ready-to-usedye composition in accordance with the invention are not, or are notsubstantially, adversely affected by the addition or additionsenvisaged.

The ready-to-use dye composition in accordance with the invention can bein various forms, such as in the form of liquids, creams or gels, whichare optionally pressurized, or in any other form which is suitable fordyeing keratin fibres, and in particular human hair. In the case of aready-to-use dye composition, the oxidation dyes(s) and the cholineoxidase are present within the same ready-to-use composition, andconsequently the said composition must be free of oxygen gas, so as toavoid any premature oxidation of the oxidation dye(s).

A subject of the invention is also a process for dyeing keratin fibres,and in particular human keratin fibres such as the hair, using theready-to-use dye composition as defined above.

According to this process, at least one ready-to-use dye composition asdefined above is applied to the fibres, for a period which is sufficientto develop the desired coloration, after which the fibres are rinsed,optionally washed with shampoo, rinsed again and dried.

The time required to develop the coloration on the keratin fibres isgenerally between 3 and 60 minutes and even more precisely between 5 and40 minutes.

According to one specific embodiment of the invention, the processincludes a first step which consists in separately storing, on the onehand, a composition (A) comprising, in a medium which is suitable fordyeing, at least one oxidation base and, on the other hand, acomposition (B) containing, in a medium which is suitable for dyeing,choline oxidase in the presence of at least one donor of the saidcholine oxidase, and then in mixing them together at the time of use,before applying this mixture to the keratin fibres.

Another subject of the invention is a multi-compartment dyeing device or"kit" or any other multi-compartment packaging system, a firstcompartment of which contains composition (A) as defined above and asecond compartment of which contains composition (B) as defined above.These devices can be equipped with means for applying the desiredmixture to the hair, such as the devices described in patentFR-2,586,913 in the name of the Applicant.

The examples which follow are intended to illustrate the inventionwithout, however, limiting its scope.

EXAMPLES Comparative Examples 1 and 2

The ready-to-use dye compositions below were prepared (contents ingrams):

    ______________________________________                                        COMPOSITION          1        2(*)                                            ______________________________________                                        para-Phenylenediamine (oxidation                                                                   0.216    0.216                                           base)                                                                         2,4-Diaminophenoxyethanol                                                                          0.723    0.723                                           dihydrochloride (coupler)                                                     Choline oxidase from 1.111    --                                              Alcaligenes, at a concentration                                               of 18 International Units                                                     (I.U.)/mg, sold by the company                                                Sigma (enzyme in accordance with                                              the invention)                                                                Choline hydrochloride (donor in                                                                    0.166    --                                              accordance with the invention)                                                Uricase from Arthobacter                                                                           --       1.0                                             globiformis, at a concentration                                               of 20 International Units                                                     (I.U.)/mg, sold by the company                                                Sigma (enzyme not in accordance                                               with the invention)                                                           Uric acid (donor not in                                                                            --       0.2                                             accordance with the invention)                                                Ethanol              10.0     10.0                                            Monoethanolamine qs  pH = 9.5 pH = 9.5                                        Demineralized water qs                                                                             100 g    100 g                                           ______________________________________                                         (*) Example not forming part of the invention                            

It is important to note that each of the ready-to-use dye compositionsdescribed above contain the same amount of enzyme, i.e. 20,000 I.U.

Each of the ready-to-use dye compositions described above was applied tolocks of permed grey hair containing 90% white hairs, for 30 minutes.The hair was then rinsed, washed with a standard shampoo and then dried.

The colour of the locks was then evaluated, before and 24 hours afterthe dyeing operation, in the Munsell system using a Minolta CM 2002®calorimeter so as to determine the strength of the colorations obtainedwith each of the compositions described above.

The difference between the colour of a lock before dyeing and the colourof the lock after dyeing was calculated by applying the Nickersonformula

    ΔE=0.4CoΔH+6ΔV+3ΔC

as described, for example, in "Couleur, Industrie et Technique": pages14-17; vol No. 5; 1978.

In this formula, ΔE represents the colour difference between two locks,ΔH, ΔV and ΔC represent the variation in absolute value of theparameters H, V and C, and Co represents the purity of the lock relativeto which it is desired to evaluate the colour difference.

The strength of the coloration (ΔE) is proportionately greater thehigher the figure indicated.

The results are given in Table I below:

                  TABLE I                                                         ______________________________________                                        Colour of the Colour of the                                                                            Strength of the                                      hair before   hair after coloration                                           EXAMPLE dyeing    dyeing     ΔH                                                                           ΔV                                                                           ΔC                                                                          ΔE                           ______________________________________                                        1       4.2 Y 5.6/1.6                                                                           8.6 PB 2.6/2.4                                                                           45.6 3.0  0.8 49.6                               2(*)    4.2 Y 5.6/1.6                                                                           8.9 PB 2.9/1.6                                                                           45.3 2.7  0   45.2                               ______________________________________                                         (*): Example not forming part of the invention                           

These results show that the ready-to-use dye composition of Example 1 inaccordance with the invention, i.e. the composition containing, asoxidizing system, the combination of choline oxidase and choline, leadsto a coloration which is stronger than that obtained with theready-to-use dye composition of Example 2 not forming part of theinvention since it contains, as oxidizing system, the combination ofuricase and uric acid. The use of the uricase/uric acid oxidizing systemis described in particular in patent application EP-A-0,310,675.

What is claimed is:
 1. A ready-to-use composition for the oxidationdyeing of keratin fibres wherein said composition comprises:at least oneoxidation base, at least one choline oxidase, and at least one donor forsaid at least one choline oxidase.
 2. The ready-to-use composition ofclaim 1 wherein said keratin fibers are human keratin fibers.
 3. Theready-to-use composition of claim 2 wherein said human keratin fibersare hair.
 4. The ready-to-use composition of claim 1, wherein said atleast one oxidation base is chosen from para-phenylenediamines, doublebases, para-aminophenols, ortho-aminophenols and heterocyclic oxidationbases.
 5. The ready-to-use composition of claim 4, wherein saidpara-phenylenediamines are chosen from compounds of formula (I) and acidaddition salts thereof: ##STR7## in which: R₁ is chosen from a hydrogenatom, C₁ -C₄ alkyl radicals, C₁ -C₄ monohydroxyalkyl radicals, C₂ -C₄polyhydroxyalkyl radicals, (C₁ -C₄)alkoxy(C₁ -C₄)alkyl radicals and C₁-C₄ alkyl radicals substituted with an entity chosen from nitrogenousgroups, phenyl radicals and 4'-aminophenyl radicals;R₂ is chosen from ahydrogen atom, C₁ -C₄ alkyl radicals, C₁ -C₄ monohydroxyalkyl radicals,C₂ -C₄ polyhydroxyalkyl radicals, (C₁ -C₄)alkoxy(C₁ -C₄)alkyl radicalsand C₁ -C₄ alkyl radicals substituted with a nitrogenous group; R₃ ischosen from a hydrogen atom, halogen atoms, C₁ -C₄ alkyl radicals, C₁-C₄ monohydroxyalkyl radicals, C₁ -C₄ hydroxyalkoxy radicals,acetylamino(C₁ -C₄)alkoxy radicals, C₁ -C₄ mesylaminoalkoxy radicals andcarbamoylamino(C₁ -C₄)alkoxy radicals; R₄ is chosen from a hydrogenatom, halogen atoms and C₁ -C₄ alkyl radicals.
 6. The ready-to-usecomposition of claim 5, wherein said nitrogenous group(s) is (are)chosen from amino radicals, mono(C₁ -C₄)alkylamino radicals, di(C₁-C₄)alkylamino radicals, tri(C₁ -C₄)alkylamino radicals, monohydroxy(C₁-C₄)alkylamino radicals, imidazolinium radicals and ammonium radicals.7. The ready-to-use composition of claim 5, wherein said halogen atomsare chosen from chlorine, bromine, iodine and fluorine atoms.
 8. Theready-to-use composition of claim 4, wherein said para-phenylenediaminesare chosen from para-phenylenediamine, para-toluylenediamine,2-chloro-para-phenylenediamine, 2,3-dimethyl-para-phenylenediamine,2,6-dimethyl-para-phenylenediamine, 2,6-diethyl-para-phenylenediamine,2,5-dimethyl-para-phenylenediamine, N,N-dimethyl-para-phenylenediamine,N,N-diethyl-para-phenylenediamine, N,N-dipropyl-para-phenylenediamine,4-amino-N,N-diethyl-3-methylaniline,N,N-bis(β-hydroxyethyl)-para-phenylenediamine,4-amino-N,N-bis(β-hydroxyethyl)-2-methylaniline,4-amino-2-chloro-N,N-bis(β-hydroxyethyl)aniline,2-β-hydroxyethyl-para-phenylenediamine, 2-fluoro-para-phenylenediamine,2-isopropyl-para-phenylenediamine,N-(β-hydroxypropyl)-para-phenylenediamine,2-hydroxymethyl-para-phenylenediamine,N,N-dimethyl-3-methyl-para-phenylenediamine,N,N-(ethyl-β-hydroxyethyl)-para-phenylenediamine,N-(β,γ-dihydroxypropyl)-para-phenylenediamine,N-(4'-aminophenyl)-para-phenylenediamine,N-phenyl-para-phenylenediamine,2-β-hydroxyethyloxy-para-phenylenediamine,2-β-acetylaminoethyloxy-para-phenylenediamine,N-(β-methoxyethyl)-para-phenylenediamine, and acid addition saltsthereof.
 9. The ready-to-use composition of claim 8, wherein saidpara-phenylenediamines are chosen from para-phenylenediamine,para-toluylenediamine, 2-isopropyl-para-phenylenediamine,2-β-hydroxyethyl-para-phenylenediamine,2-β-hydroxyethyloxy-para-phenylenediamine,2,6-dimethyl-para-phenylenediamine, 2,6-diethyl-para-phenylenediamine,2,3-dimethyl-para-phenylenediamine,N,N-bis(β-hydroxyethyl)-para-phenylenediamine,2-chloro-para-phenylenediamine,2-β-acetylaminoethyloxy-para-phenylenediamine, and acid addition saltsthereof.
 10. The ready-to-use composition of claim 4, wherein saiddouble bases are chosen from compounds corresponding to formula (II) andacid addition salts thereof: ##STR8## in which: Z₁ and Z₂, which may beidentical or different, are chosen from a hydroxyl radical, an --NH₂radical which may be substituted with a substituent chosen from C₁ -C₄alkyl radicals and linker arms Y;the linker arm Y is chosen from linearand branched alkylene chains containing from 1 to 14 carbon atoms, whichmay be interrupted by or terminated with at least one entity chosen fromnitrogenous groups and hetero atoms, and optionally substituted with atleast one substituent chosen from hydroxyl and C₁ -C₆ alkoxy radicals;R₅ and R₆ are chosen from a hydrogen atom, halogen atoms, C₁ -C₄ alkylradicals, C₁ -C₄ monohydroxyalkyl radicals, C₂ -C₄ polyhydroxyalkylradicals, C₁ -C₄ aminoalkyl radicals and linker arms Y; R₇, R₈, R₉, R₁₀,R₁₁ and R₁₂, which may be identical or different, are chosen from ahydrogen atom, linker arms Y, and C₁ -C₄ alkyl radicals;it beingunderstood that the compounds of formula (II) contain only one linkerarm Y per molecule.
 11. The ready-to-use composition of claim 10,wherein said heteroatoms are chosen from oxygen, sulphur and nitrogenatoms.
 12. The ready-to-use composition of claim 10, wherein saidnitrogenous groups are chosen from amino radicals, mono(C₁-C₄)alkylamino radicals, di(C₁ -C₄)alkylamino radicals, tri(C₁-C₄)alkylamino radicals, monohydroxy(C₁ -C₄)alkylamino radicals,imidazolinium radicals and ammonium radicals.
 13. The ready-to-usecomposition of claim 4, wherein said double bases are chosen fromN,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)-1,3-diaminopropanol,N,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)ethylenediamine,N,N'-bis(4-aminophenyl)tetramethylenediamine,N,N'-bis(β-hydroxyethyl)-N,N'-bis(4-aminophenyl)tetramethylenediamine,N,N'-bis(4-methylaminophenyl)tetramethylenediamine,N,N'-bis(ethyl)-N,N'-bis(4'-amino-3'-methylphenyl)ethylenediamine and1,8-bis(2,5-diaminophenoxy)-3,5-dioxaoctane, and acid addition saltsthereof.
 14. The ready-to-use composition of claim 13, wherein saiddouble bases are chosen fromN,N'-bis(β-hydroxyethyl)-N,N'-bis(4'-aminophenyl)-1,3-diaminopropanol,1,8-bis(2,5-diaminophenoxy)-3,5-dioxaoctane, and acid addition saltsthereof.
 15. The ready-to-use composition of claim 4, wherein saidpara-aminophenols are chosen from compounds corresponding to formula(III) and acid addition salts thereof: ##STR9## in which: R₁₃ is chosenfrom a hydrogen atom, halogen atoms, C₁ -C₄ alkyl radicals, C₁ -C₄monohydroxyalkyl radicals, (C₁ -C₄)alkoxy(C₁ -C₄)alkyl radicals, C₁ -C₄aminoalkyl radicals and hydroxy(C₁ -C₄)alkylamino(C₁ -C₄)alkylradicals,R₁₄ is chosen from a hydrogen atom, halogen atoms, C₁ -C₄ alkylradicals, C₁ -C₄ monohydroxyalkyl radicals, C₂ -C₄ polyhydroxyalkylradicals, C₁ -C₄ aminoalkyl radicals, C₁ -C₄ cyanoalkyl radicals and (C₁-C₄)alkoxy-(C₁ -C₄)alkyl radicals,it being understood that at least oneof the radicals R₁₃ or R₁₄ represents a hydrogen atom.
 16. Theready-to-use composition of claim 4, wherein said para-aminophenols arechosen from para-aminophenol, 4-amino-3-methylphenol,4-amino-3-fluorophenol, 4-amino-3-(hydroxymethyl)phenol,4-amino-2-methylphenol, 4-amino-2-(hydroxymethyl)phenol,4-amino-2-(methoxymethyl)phenol, 4-amino-2-(aminomethyl)-phenol,4-amino-2-((β-hydroxyethyl)aminomethyl)phenol, 4-amino-2-fluorophenoland acid addition salts thereof.
 17. The ready-to-use composition ofclaim 4, wherein said ortho-aminophenols are chosen from 2-aminophenol,2-amino-5-methylphenol, 2-amino-6-methylphenol,5-acetamido-2-aminophenol and acid addition salts thereof.
 18. Theready-to-use composition of claim 4, wherein said heterocyclic bases arechosen from pyridines, pyrimidines, pyrazoles, pyrazolopyrimidines andacid addition salts thereof.
 19. The ready-to-use composition of claim18, wherein said pyrazolopyrimidines are chosen from compounds offormula (IV), acid addition salts thereof, base addition salts thereof,and tautomeric forms thereof, when there exists a tautomericequilibrium: ##STR10## in which: R₁₅, R₁₆, R₁₇ and R₁₈, which areidentical or different, are chosen from a hydrogen atom, C₁ -C₄ alkylradicals, aryl radicals, C₁ -C₄ hydroxyalkyl radicals, C₂ -C₄polyhydroxyalkyl radicals, (C₁ -C₄)alkoxy(C₁ -C₄)alkyl radicals, C₁ -C₄aminoalkyl radicals wherein the amine may be protected by an acetyl,ureido or sulphonyl radical, (C₁ -C₄)alkylamino(C₁ -C₄)alkyl radicals,di((C₁ -C₄)alkyl)amino(C₁ -C₄)alkyl radicals wherein the dialkylradicals may form a ring chosen from 5- and 6- membered carbon-based andheterocyclic rings, hydroxy(C₁ -C₄)alkylamino(C₁ -C₄)alkyl radicals, anddi(hydroxy(C₁ -C₄)alkyl)amino(C₁ -C₄)alkyl radicals;the X radicals,which are identical or different, are chosen from a hydrogen atom, C₁-C₄ alkyl radicals, aryl radicals, C₁ -C₄ hydroxyalkyl radicals, C₂ -C₄polyhydroxyalkyl radicals, C₁ -C₄ aminoalkyl radicals, (C₁-C₄)alkylamino(C₁ -C₄)alkyl radicals, di((C₁ -C₄)alkyl)amino(C₁-C₄)alkyl radicals wherein the dialkyls may form a ring chosen from 5-and 6-membered carbon-based and heterocyclic rings, hydroxy(C₁-C₄)alkylamino(C₁ -C₄)alkyl radicals, di(hydroxy(C₁ -C₄)alkyl)-amino(C₁-C₄)alkyl radicals, amino radicals, (C₁ -C₄)alkylamino radicals, di((C₁-C₄)alkyl)amino radicals, halogen atoms, carboxylic acid groups andsulphonic acid groups; i has the value 0, 1, 2 or 3; p has the value 0or 1; q has the value 0 or 1; n has the value 0 or 1;with the provisothat: the sum p+q is other than 0; when p+q is equal to 2, then n hasthe value 0 and the NR₁₅ R₁₆ and NR₁₇ R₁₈ groups occupy the (2,3),(5,6), (6,7), (3,5) or (3,7) positions; when p+q is equal to 1, then nhas the value 1 and the NR₁₅ R₁₆ or NR₁₇ R₁₈ group and the OH groupoccupy the (2,3), (5,6), (6,7), (3,5) or (3,7) positions.
 20. Theready-to-use composition of claim 19, wherein said compounds of formula(IV) are chosen frompyrazolo(1,5-a)pyrimidine-3,7-diamine;2,5-dimethylpyrazolo(1,5-a)pyrimidine-3,7-diamine;pyrazolo(1,5-a)pyrimidine-3,5-diamine; 2.7-dimethylpyrazolo(1,5-a)pyrimidine-3,5-diamine;3-aminopyrazolo(1,5-a)pyrimidin-7-ol;3-aminopyrazolo(1,5-a)pyrimidin-5-ol;2-(3-aminopyrazolo(1,5-a)pyrimidin-7-ylamino)ethanol;2-(7-aminopyrazolo(1,5-a)pyrimidin-3-ylamino)ethanol;2-((3-aminopyrazolo(1,5-a)pyrimidin-7-yl)-(2-hydroxyethyl)amino)ethanol;2-((7-aminopyrazolo(1,5-a)pyrimidin-3-yl)-(2-hydroxyethyl)amino)ethanol;5,6-dimethylpyrazolo(1,5-a)pyrimidine-3,7-diamine;2,6-dimethylpyrazolo(1,5-a)pyrimidine-3,7-diamine;2,5,N7,N7-tetramethylpyrazolo(1,5-a)pyrimidine-3,7-diamine;acid additionsalts thereof, base addition salts thereof, and tautomeric formsthereof, when there exists a tautomeric equilibrium.
 21. Theready-to-use composition of claim 1, wherein said at least one oxidationbase is present in an amount ranging from 0.0005 to 12% by weightrelative to the total weight of the composition.
 22. The ready-to-usecomposition of claim 21, wherein said at least one oxidation base ispresent in an amount ranging from 0.005 to 6% by weight relative to thetotal weight of the composition.
 23. The ready-to-use composition ofclaim 1, wherein said at least one choline oxidase is chosen from thoseof animal, microbiological, and synthetic origin.
 24. The ready-to-usecomposition of claim 1, wherein said at least one choline oxidase ispresent in an amount ranging from 0.01 to 20% by weight relative to thetotal weight of the composition.
 25. The ready-to-use composition ofclaim 24, wherein said at least one choline oxidase is present in anamount ranging from 0.1 to 5% by weight relative to the total weight ofthe composition.
 26. The ready-to-use composition of claim 1, whereinsaid at least one donor is chosen from choline, acid addition salts ofcholine, and betaine aldehyde.
 27. The ready-to-use composition of claim1, wherein said at least one donor is present in an amount ranging from0.01 to 20% by weight relative to the total weight of the composition.28. The ready-to-use composition of claim 27, wherein said at least onedonor is present in an amount ranging from 0.1 to 5% by weight relativeto the total weight of the composition.
 29. The ready-to-use compositionof claim 1, wherein said composition further comprises at least oneadditional ingredient chosen from couplers and direct dyes.
 30. Theready-to-use composition of claim 29, wherein said couplers are chosenfrom meta-phenylenediamines, meta-aminophenols, meta-diphenols,heterocyclic couplers and the acid addition salts thereof.
 31. Theready-to-use composition of claim 29, wherein said couplers are chosenfrom indoles, indolines, benzimidazoles, benzomorpholines, sesamols,pyridines, pyrimidines, and pyrazoles, and the acid addition saltsthereof.
 32. The ready-to-use composition of claim 29, wherein saidcouplers are chosen from 2-methyl-5-aminophenol,5-N-(β-hydroxyethyl)amino-2-methylphenol, 3-aminophenol,1,3-dihydroxybenzene, 1,3-dihydroxy-2-methylbenzene,4-chloro-1,3-dihydroxybenzene, 2,4-diamino-1-(β-hydroxyethyloxy)benzene,2-amino-4-(β-hydroxyethylamino)-1-methoxybenzene, 1,3-diaminobenzene,1,3-bis(2,4-diaminophenoxy)propane, sesamol, α-naphthol,6-hydroxyindole, 4-hydroxyindole, 4-hydroxy-N-methylindole,6-hydroxyindoline, 2,6-dihydroxy-4-methylpyridine,1H-3-methylpyrazol-5-one, 1-phenyl-3-methylpyrazol-5-one, and the acidaddition salts thereof.
 33. The ready-to-use composition of claim 29,wherein said couplers are present in an amount ranging from 0.0001 to10% by weight relative to the total weight of the composition.
 34. Theready-to-use composition of claim 33, wherein said couplers are presentin an amount ranging from 0.005 to 5% by weight relative to the totalweight of the composition.
 35. The ready-to-use composition of claim 1,wherein said composition further comprises a medium suitable for dyeing,said medium comprising water or a mixture of water and at least oneorganic solvent .
 36. The ready-to-use composition of claim 35, whereinsaid at least one organic solvent is chosen from C₁ -C₄ alkanols,glycerol, glycols, glycol ethers and aromatic alcohols.
 37. Theready-to-use composition of claim 35, wherein said at least one organicsolvent is present in an amount ranging from 1 to 40% by weight relativeto the total weight of the composition.
 38. The ready-to-use compositionof claim 37, wherein said at least one organic solvent is present in anamount ranging from 5 to 30% relative to the total weight of thecomposition.
 39. The ready-to-use composition of claim 1, wherein saidcomposition has a pH ranging from 5 to
 11. 40. The ready-to-usecomposition of claim 39, wherein said pH ranges from 6.5 to
 10. 41. Theready-to-use composition of claim 1, wherein said composition is in theform of a liquid, a cream, a gel or any other form appropriate fordyeing keratin fibers.
 42. The ready-to-use composition of claim 1,wherein said at least one oxidation base and said at least one cholineoxidase are present within the same ready-to-use composition and saidcomposition is free of oxygen gas.
 43. A method for dyeing keratinfibres, comprising the steps of contacting said fibres for a timesufficient to achieve color development, with a ready-to-use dyecomposition comprising:at least one oxidation base, at least one cholineoxidase, and at least one donor for said at least one choline oxidase.44. The method of claim 43, wherein said keratin fibers are humankeratin fibers.
 45. The method of claim 44, wherein said human keratinfibers are hair.
 46. The method of claim 43, wherein said timesufficient ranges from 3 to 60 minutes.
 47. The method of claim 46,wherein said time sufficient ranges from 5 to 40 minutes.
 48. A methodfor dyeing keratin fibres, comprising separately storing a firstcomposition comprising at least one oxidation base and a secondcomposition containing at least one choline oxidase in the presence ofat least one donor of said at least one choline oxidase, and thereaftermixing said first composition with said second composition and applyingthis mixture to the keratin fibres.
 49. A multi-compartment dyeingdevice or kit for dyeing keratin fibers, comprising at least twoseparate compartments, whereina first compartment contains a compositioncomprising at least one oxidation base and a second compartment containsa composition comprising at least one choline oxidase in the presence ofat least one donor of said choline oxidase.